You should be doing the injection in a glovebox, or at the very least, a still-air environment. If you are going to work in open air, we suggest turning your AC/heater off an hour prior to inoculating your bags (this reduces the amount of airborne contaminants in the area). It is also recommended to work in a clean, small room with the door closed to prevent airflow.
Step 1: The first step of inoculating a grain bag is to prepare it for injection with spores or mycelial culture. Carefully pull the gussets on the bag apart all the way up to the top of the bag, where it is sealed. You want to make sure to "inflate" the bag enough so the filter has some room to "breathe." If the filter is pressed up against the plastic of the bag, it will limit gas exchange during the colonization phase and could potentially stall the colonization process.
Step 5: Place your bag in a place that is subject to minimum disturbances and will maintain a temperature between 75 and 81°F. It doesn't matter if it is dark or light. You should see signs of growth 3-10 days after inoculation. Try your best to disturb the bag as little as possible during this time period. You want the bag in an upright position (not rolled up) with ample space between the filter and the plastic of the bag so it can breathe during this critical colonization time period. See picture above.
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